mouse anti βiii tubulin  (Santa Cruz Biotechnology)

Journal: Journal of neuroinflammation

Article Title: Targeted activation of ErbB4 receptor ameliorates neuronal deficits and neuroinflammation in a food-borne polystyrene microplastic exposed mouse model.

doi: 10.1186/s12974-025-03406-6

Figure Lengend Snippet: Fig. 3 ErbB4 small molecule agonist can improve synaptic dysfunction of hippocampal neurons in mice exposed to PS-MPs. (A) The ultrastructural analysis of hippocampal neuron synapses was performed using transmission electron microscopy (TEM). (B) Statistical analyses were conducted on the synaptic data of hippocampal neurons. (C) Immunofluorescence was employed to detect the levels of synaptophysin (SYP) and β3-tubulin. (D) Western blot analysis was utilized to assess the protein levels of synapse-associated proteins, including DOCK3, p-CREB, CREB, SYP, GAP43, PSD95, and SYT1 in hippocampal neurons. (E) Statistical analyses of these protein levels were performed using one-way ANOVA with Tukey’s multiple comparison test. The synaptic structure was examined at a magnification of 10,000×, with a scale bar of 1 μm. Results are expressed as means ± SEM. Immunofluorescence labeling was as follows: SYP in red and β3-tubulin in green, with a scale bar of 20 μm. Statistical significance was indicated by *p < 0.05, **p < 0.01, and ***p < 0.001, with a sample size of n = 4 per group

Article Snippet: Specifically, the sections were treated with a mouse anti-β3-tubulin antibody (1:100, sc80016, Santa Cruz Biotechnology, USA) in combination with one of the following antibodies: rabbit anti-p-ErbB4 antibody (1:200, bs-3220R, Bioss, Beijing, China), and rabbit antisynaptophysin (SYP) antibody (1:200, AF8091, Beyotime, Shanghai, China).

Techniques: Transmission Assay, Electron Microscopy, Immunofluorescence, Western Blot, Comparison, Labeling

Journal: Journal of neuroinflammation

Article Title: Targeted activation of ErbB4 receptor ameliorates neuronal deficits and neuroinflammation in a food-borne polystyrene microplastic exposed mouse model.

doi: 10.1186/s12974-025-03406-6

Figure Lengend Snippet: Fig. 5 ErbB4 small molecule agonist enhances the ErbB4 signaling pathway in hippocampus of mice exposed to PS-MPs. (A) Immunofluorescence was used to detect levels of phosphorylated ErbB4 (p-ErbB4) and β3-tubulin. (B) Protein levels associated with the ErbB4 signaling pathway in the hippo campus of mice were assessed through western blot analysis. (C) A statistical analysis was performed on the data derived from protein levels, employing one-way ANOVA with Tukey’s multiple comparison tests. The p-ErbB4 is represented in red, while β3-tubulin is depicted in green, with a scale bar of 20 μm. The results are expressed as means ± SEM, with significance levels indicated by *p < 0.05, **p < 0.01, and ***p < 0.001. Each experimental group comprised n = 4 samples

Article Snippet: Specifically, the sections were treated with a mouse anti-β3-tubulin antibody (1:100, sc80016, Santa Cruz Biotechnology, USA) in combination with one of the following antibodies: rabbit anti-p-ErbB4 antibody (1:200, bs-3220R, Bioss, Beijing, China), and rabbit antisynaptophysin (SYP) antibody (1:200, AF8091, Beyotime, Shanghai, China).

Techniques: Immunofluorescence, Western Blot, Derivative Assay, Comparison